Motivation is the driving force that can make an employee efficient at work. Women’s self-motivation can play a significant role in their professional success. Moreover, self-motivation to perform multiple responsibilities at work has major impact on women’s representation in process of decision-making. Women actively take part into various sectors of economy but are poorly represented at decision making positions. In medical field, bunch of females are working side by side with male. However, their role, particularly at decision making positions is limited across the world. Therefore, this research has focused on analyzing the extent to which women participate at decision making positions in medical field with special focus on public sector medical institutions of Pakistan. Therefore, one of the leading public sector medical universities i.e. Liaquat University of Medical & Health Sciences, Jamshoro has been selected as sample medical institute in Pakistan to present the findings. Study results confirmed that women’s self-motivation has significant impact on their representation in decision making position.